Overview
NAD+ (nicotinamide adenine dinucleotide) is a dinucleotide coenzyme composed of a nicotinamide mononucleotide and an adenine mononucleotide joined through their phosphate groups. It is a central electron carrier in cellular metabolism, cycling between its oxidized (NAD+) and reduced (NADH) forms. Beyond redox chemistry, NAD+ is also consumed as a substrate by several enzyme families. It is supplied here as a research-grade reagent rather than as a therapeutic preparation.
Mechanism as studied
The research literature describes NAD+ in two broad roles. As a redox coenzyme it accepts and donates hydride equivalents across glycolysis, the citric-acid cycle, and oxidative phosphorylation via the NAD+/NADH couple. As a consumed co-substrate it is characterized as obligate for the sirtuin family of NAD+-dependent deacylases and for poly(ADP-ribose) polymerases (PARPs), linking cellular NAD+ availability to DNA-repair signaling, chromatin regulation, and mitochondrial function. These roles are documented at the enzymatic and cellular level.
Research context
Contexts in which this compound appears in the in-vitro and preclinical research literature:
- Enzyme assays of sirtuin (NAD+-dependent deacylase) activity as a function of NAD+ concentration.
- PARP-mediated poly-ADP-ribosylation and DNA-damage-response studies in cell lysates.
- Redox-balance measurements of the NAD+/NADH ratio in cultured cells.
- Preclinical models of metabolic and neurodegenerative dysfunction examining intracellular NAD+ pools.
- Studies of NAD+ salvage and biosynthetic pathways using nicotinamide and related precursors.
Handling & storage
Handled as a research reagent, NAD+ is supplied as a powder that dissolves readily in water or an appropriate aqueous buffer to the concentration required by the assay. Because NAD+ in solution degrades over time, particularly at neutral-to-alkaline pH, prepare working solutions fresh and keep them chilled. Aliquot stock to avoid repeated freeze–thaw.
Store the sealed powder at −20 °C, protected from light and moisture, where it is stable long-term. Aqueous solutions should be prepared fresh, kept cold, and not repeatedly frozen and thawed.
Certificates of analysis
Independent and in-house certificates published for this compound. Each reports identity and HPLC purity for the tested lot.
Order NAD+
Lot-traceable, certificate-backed, and shipped cold-chain from the US.
For research use only
This guide is reference material for qualified researchers. The compound is an analytical-grade reference material supplied strictly for in-vitro laboratory research and development — not a drug, supplement, or medical device, and not for human or animal consumption or any therapeutic, clinical, or diagnostic use. No medical claims are made or implied.
