Overview
5-Amino-1MQ (5-amino-1-methylquinolinium) is a small-molecule, cell-permeable inhibitor of nicotinamide N-methyltransferase (NNMT), an enzyme overexpressed in adipose and other tissues under metabolic stress. It is not a peptide; it is a low-molecular-weight quinolinium compound studied as a selective NNMT reference inhibitor. Because NNMT normally consumes nicotinamide and S-adenosylmethionine (SAM), its inhibition is examined for downstream effects on cellular methylation and NAD+ salvage pathways.
Mechanism as studied
The research literature characterizes 5-Amino-1MQ as a competitive inhibitor that occupies the nicotinamide-binding site of NNMT. By blocking methylation of nicotinamide, in-vitro studies describe preservation of intracellular NAD+ and SAM pools and reduction of the reaction product 1-methylnicotinamide. In cultured adipocytes and rodent models of diet-induced obesity, this shift is associated with reduced adipocyte size and altered insulin- and energy-metabolism signaling.
Research context
Contexts in which this compound appears in the in-vitro and preclinical research literature:
- Cultured adipocyte models: NNMT inhibition with NAD+ and SAM pool measurements.
- Rodent models of diet-induced obesity examining fat-mass and metabolic endpoints.
- Enzyme-kinetics assays characterizing NNMT active-site inhibition.
- Studies of 1-methylnicotinamide as a downstream reaction product.
- Insulin-signaling and energy-expenditure endpoints in preclinical models.
Handling & storage
Supplied as a powder for laboratory reconstitution. As a small-molecule salt it is prepared in an appropriate laboratory solvent per its solubility (commonly aqueous buffer or bacteriostatic water; DMSO stock solutions are used for some assay formats) at the working concentration your protocol requires. Mix gently to fully dissolve and aliquot stock solutions to limit freeze–thaw exposure.
Store the sealed vial at −20 °C protected from light. Keep reconstituted stock solutions refrigerated or frozen per your protocol and minimize repeated freeze–thaw cycles.
Certificates of analysis
Independent and in-house certificates published for this compound. Each reports identity and HPLC purity for the tested lot.
Order 5-Amino-1MQ
Lot-traceable, certificate-backed, and shipped cold-chain from the US. Available in 10mg, 50mg.
For research use only
This guide is reference material for qualified researchers. The compound is an analytical-grade reference material supplied strictly for in-vitro laboratory research and development — not a drug, supplement, or medical device, and not for human or animal consumption or any therapeutic, clinical, or diagnostic use. No medical claims are made or implied.
