Overview
TB-500 is a synthetic peptide reproducing the central actin-binding region of thymosin β-4 (Tβ4), a 43-amino-acid regulatory peptide expressed across many mammalian cell types. The fragment corresponds to the conserved motif through which Tβ4 engages monomeric actin, and it is produced synthetically rather than isolated from tissue. Unlike the full-length parent peptide, it represents only the actin-interacting active region and is supplied as a water-soluble powder for research use. It is frequently studied as a reference peptide in cytoskeletal-dynamics and cell-migration assays.
Mechanism as studied
The research literature characterizes TB-500 principally as a G-actin-sequestering peptide. By binding monomeric (globular) actin, it is described as buffering the pool of polymerization-competent actin and thereby influencing the equilibrium between G-actin and filamentous F-actin during cytoskeletal remodeling. In cultured endothelial and epithelial systems, this actin-regulating activity is associated with cell migration, spreading, and angiogenic sprouting endpoints. Additional in-vitro work describes modulation of genes linked to cell motility and downregulation of certain inflammatory mediators.
Research context
Contexts in which this compound appears in the in-vitro and preclinical research literature:
- Cytoskeletal assays: G-actin binding and the G-/F-actin equilibrium under actin-polymerization endpoints.
- Endothelial migration and tube-formation models examining angiogenic sprouting.
- Epithelial and fibroblast wound-closure (scratch) assays measuring cell motility.
- Preclinical rodent studies referenced in the literature examining cardiac, corneal, and connective-tissue endpoints.
- Comparative studies against full-length thymosin β-4 characterizing the isolated actin-binding fragment.
Handling & storage
Supplied as a lyophilized powder for reconstitution in the laboratory, typically with bacteriostatic water; the diluent volume is chosen to reach the working concentration the assay requires (e.g. adding 2 mL to a 10 mg vial yields 5 mg/mL). Add diluent slowly down the vial wall and swirl gently to dissolve; do not vortex or shake. Prepare in a clean field and aliquot to minimize freeze–thaw cycles.
Store the sealed lyophilized vial at −20 °C protected from light, where it is stable long-term. Once reconstituted, keep refrigerated at 2–8 °C and use within the assay window documented for your protocol, avoiding repeated freeze–thaw.
Certificates of analysis
Independent and in-house certificates published for this compound. Each reports identity and HPLC purity for the tested lot.
Order TB-500
Lot-traceable, certificate-backed, and shipped cold-chain from the US.
For research use only
This guide is reference material for qualified researchers. The compound is an analytical-grade reference material supplied strictly for in-vitro laboratory research and development — not a drug, supplement, or medical device, and not for human or animal consumption or any therapeutic, clinical, or diagnostic use. No medical claims are made or implied.
